Polynucleotides. XXIII(1). A synthesis of ribodinucleoside monophosphates using nucleoside 5′-phosphates
نویسندگان
چکیده
منابع مشابه
Formation of mixed-ligand complexes of Pd2+ with nucleoside 5'-monophosphates and some metal-ion-binding nucleoside surrogates.
Formation of mixed-ligand Pd2+ complexes between canonical nucleoside 5'-monophosphates and five metal-ion-binding nucleoside analogs has been studied by 1H-NMR spectroscopy to test the ability of these nucleoside surrogates to discriminate between unmodified nucleobases by Pd2+-mediated base pairing. The nucleoside analogs studied included 2,6-bis(3,5-dimethylpyrazol-1-yl)-, 2,6-bis(1-methylhy...
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15 صفحه اولSynthesis of γ-labeled nucleoside 5'-triphosphates using click chemistry.
Real-time enzymatic studies are gaining importance as their chemical and technical instrumentation improves. Here we report the efficient synthesis of γ-alkyne modified triphosphate amidates that are converted into a variety of γ-fluorophore labeled triphosphates by Cu(I) catalyzed alkyne/azide click reactions. The synthesized triphosphates are incorporated into DNA by DNA polymerases.
متن کاملEnzymatic Synthesis of Polynucleotides
Several publications from this laboratory have dealt in a preliminary fashion with the preparation and properties of highly purified polynucleotide phosphorylase from Azotobacter vinelan&i (2-4). A more detailed account of this work is presented in thii paper. Although polynucleotide phosphorylase preparations can catalyze the synthesis of a variety of homopolymers (polyadenylic acid, polyuridy...
متن کاملEnzymatic Synthesis of Polynucleotides
Several publications from this laboratory have dealt in a preliminary fashion with the preparation and properties of highly purified polynucleotide phosphorylase from Azotobacter vinelan&i (2-4). A more detailed account of this work is presented in thii paper. Although polynucleotide phosphorylase preparations can catalyze the synthesis of a variety of homopolymers (polyadenylic acid, polyuridy...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1974
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/1.2.323